In natural habitats, plants frequently experience rapid changes in the intensity of sunlight. To cope with these changes and maximize growth, plants adjust photosynthetic light utilization in electron transport and photoprotective mechanisms. This involves a proton motive force (PMF) across the thylakoid membrane, postulated to be affected by unknown anion (Cl−) channels. Ion channel activities across chloroplast envelopes and thylakoid membranes have been demonstrated, and are postulated to play critical roles in chloroplast physiology. Light-induced charge separation and coupled H+ uptake into the thylakoid lumen generate a proton motive force (PMF), composed of the transmembrane electric-potential gradient and H+ concentration gradient. Both PMF components activate and drive ATP synthesis by chloroplast F0F1 ATP synthase.
VCCN1 localizes to the thylakoid membrane, and fine-tunes PMF by anion influx into the lumen during illumination, adjusting electron transport and the photoprotective mechanisms. The activity of VCCN1 accelerates the activation of photoprotective mechanisms on sudden shifts to high light. Here you can see the inner view of VCCN1 channel structure as determined by cryoEM experiments (PDB code: 7EK3)

#molecularart ... #immolecular ... #channel ... #chloroplast ... #VCCN1 ... #cryoem ... #light ... #activation ... #ATP ... #synthesis ... #energy ... #transduction

VCCN1 channel structure rendered with @proteinimaging and depicted with @corelphotopaint

VCCN1 channel
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VCCN1 channel

Published: